GSM4770467: SKO 10D-Vector Hep; Mus musculus; RNA-Seq - SRA

SRX9084836: GSM4770467: SKO 10D-Vector Hep; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 86.6M spots, 10.7G bases, 5.3Gb downloads

Submitted by: NCBI (GEO)

Study: Cell-Autonomous Requirement of Shp2 and ß-Catenin for Myc-Driven Hepatocarcinogenesis Revealed by Single Cell RNA-Sequencing

PRJNA661754 • SRP280277 • All experiments • All runs

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We applied single-cell RNAseq analysis to characterize hepatic cell types, including hepatocytes (Hep) and non-parenchymal cells (NPC), in mouse liver, during Myc-induced hepatocellular carcinoma (HCC) development. Overall design: Hepatocytes (Hep) and Non-parenchymal cells (NPCs) were isolated from wild type (WT) and hepatocyte-specific Shp2 knockout (SKO) livers, at day 0 (0D-Ctrl) before HTVi, 10 days after Myc injection (10D-Myc), 10 days after empty vector injection (10D-Vector), and 4 weeks after Myc injection (4W-Myc).

Sample: SKO 10D-Vector Hep

SAMN16070644 • SRS7331034 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Mouse liver was perfused with collagenase H (Roche). Hepatocytes and non-parenchymal cells were separated by centrifugation. Chromium Single Cell 3' Reagent Kits (V2 chemistry, 10x Genomics)

Experiment attributes:

GEO Accession: GSM4770467

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 86.6M spots, 10.7G bases, 5.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR12600851	86,617,980	10.7G	5.3Gb	2021-12-03

ID:: 11804341

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